Effect of UV-treatment of titanium surfaces on bacterial biofilm
formation after 16 hours incubation evaluated via confocal microscopy imaging
through (a,b) 10x and (c,d) 40x objectives with
representative images illustrating the live/dead distribution of bacterial cells
(green for live cells, red for compromised cells) accumulated on
(a,c) untreated (-UV) and (b,d) UV-treated (+UV)
titanium discs. Scanning electron microscopy revealing the biofilm structure on
(e) -UV and (f) +UV titanium discs. Quantitative
comparison of (e) accumulated biomass and (f) the area
covered by bacteria between untreated (white bar, –UV) in comparison to
UV-treated (gray bar, +UV) titanium discs. Each value represents the mean
± SD of four samples comprised of two technical replicates of two
independent biological experiments. Statistically significant differences are
indicated as: * p<0.0116), # p<0.0461.