Table 1.
Properties of BphP1-FP and its Mutants Expressed in Bacteria with Either BV or PΦB
| NIR FP | Absorbance Maximum (nm) | Excitation Maximum (nm) | Emission Maximum (nm) | Extinction Coefficient (M−1 cm−1) | Quantum Yield (%) | Relative Molecular Brightness (%) |
|---|---|---|---|---|---|---|
| BphP1-FP + BV | 643 | 639 | 669 | 60,000 | 13.0 | 100 |
| BphP1-FP/C20S + BV | 642 | 639 | 670 | 82,000 | 13.9 | 146 |
| BphP1-FP/C253I + BV | 677 | 675 | 704 | 70,000 | 4.0 | 36 |
| BphP1-FP/C20S/C253I + BV | 673 | 671 | 702 | 59,000 | 3.8 | 29 |
| BphP1-FP/C20S + PΦB | 641 | 639 | 669 | 105,000 | 15.4 | 207 |
| BphP1-FP/C253I + PΦB | 677 | 675 | 703 | 59,000 | NA | NA |
NA, not available. See also Table S5.