CTS induces features of atherosclerotic plaque stability in ApoE
−/− mice. Photomicrographs of sectioned aortic sinus were stained with H&E to calculate necrotic core area (panel A, original magnification, ×100) or immunohistochemically stained for macrophage deposition (CD68 positive, panel B, original magnification, ×100), and collagen content (indicated in blue colour with Masson's trichrome staining, panel C, original magnification, ×50). Corresponding positive areas (CD68 and collagen content) were analysed and quantified using Image‐Pro Plus 6.0 software and the percentages of the above plaque components in the entire plaque were evaluated. n = 6 for each group. **P < 0.01, ***P < 0.001 compared with vehicle group respectively.