Table 2. Primers used for PCR amplification.
Oligonucleotides | Sequence (5’- 3’) | Target | Source |
---|---|---|---|
sense primer P1 | GAC ATG TCC TCC TGC ATC T | VP1 gene | This work |
antisense primer P2 | CAC AAA TGT ACA GGG ATG GGT | VP1 gene | This work |
sense primer P3 | GCC CAT GGA AAC AAC AGC AAC AG | synthetic VP1 gene | This work |
antisense primer P4 | GCT CGA GTT ATA ATA ATT GTT TCG CAG G | synthetic VP1 gene | This work |
(Restriction sites are underlined.)