Table 2. qRT PCR primers of the housekeeping gene (β-actin), KIM-1, HSP-70, ICAM-1, VCAM-1 and P-selectin primers and their sequences.
Primers | Forward | Reverse | Amplicon (bp) |
---|---|---|---|
β-actin | 5’-GGAAATCGTGCGTGACATTAAA-3’ | 5’-GCGGCAGTGGCCATCTC-3’ | 109 |
KIM-1 | 5’-AGAGAGAGCAGGACACAGGCTTT-3’ | 5’-ACCCGTGGTAGTCCCAAACA-3’ | 89 |
HSP-70 | 5’-GGTTGCATGTTCTTTGCGTTTA-3’ | 5’-GGTGGCAGTGCTGAGGTGTT-3’ | 97 |
ICAM-1 | 5’-CCAGACCCTGGAGATGGAGAA-3’ | AAGCGTCGTTTGTGATCCTCC | 251 |
VCAM-1 | 5’-TCTCTGGGTCTTCGTGTTTCTTATCT-3’ | 5’-GTGTCCCCCTAGTACCATCTGAA-3’ | 80 |
P-selectin | 5’-TGTGGAAGTGTGCCCGAAA-3’ | 5’-ACGAGCCATTAACAGACTTTAGCA-3’ | 84 |