Fig 1. c.-67G>C in the 5’UTR of F2R alters transcriptional activity.

(A) Alignment of F2R 5’UTR nucleotide sequences across multiple species, highly conserved nucleotides are highlighted in black. (B) HEK293T cells were transfected with 200 ng of either -67G or -67C F2R luciferase reporter construct, or an empty vector control. Forty-eight hours post-transfection, cells were lysed and luciferase activity assessed. Luciferase levels were normalised for transfection efficiency using Renilla activity. Data shown represents three independent experiments with a minimum of 3 technical replicates to calculate mean fold change ± SE. (*p<0.05).