Fig 3. Expression and functional analysis of WT and T22N PAR4 in HEK293 cells.
Cells were transfected with WT or T22N CFP-tagged PAR4. (A) Total CFP receptor expression was evaluated by immunofluorescence (shown in blue). Images are representative of 3 independent experiments. (C) Signalling was assessed by measuring changes in intracellular calcium concentration in response to the PAR4 peptide, AYPGKG. Data represents mean±SEM, n = 3.