Table 2. Binding parameters deduced from SPR measurements in HBS.
| Peptide a | ka (106 M-1 s-1) | kd (10−3 s-1) | KD (10−9 M) | ΔG (kcal/mol) | Number of experiments |
|---|---|---|---|---|---|
| TAMFQDPQERC | 3.05 ± 0.9 | 1.43 ± 0.05 | 0.47 ± 0.15 | -12.7 ± 0.2 | 6 |
| TAMFQDPFERC | 3.75 ± 1.15 | 1.10 ± 0.01 | 0.29 ± 0.09 | -13.0 ± 0.2 | 2 |
| TAMFQSPQERC | 4.70 | 15.00 | 3.20 | -11.6 | 1 |
| TAMFQDYQERC | 1.63 ± 0.78 | 133 ± 47 | 82 ± 68 | -9.2 ± 0.5 | 3 |
| TAMFQDVQERC | 4.20 | 31.00 | 7.40 | -11.1 | 1 |
| TAMFQDVFERC | 3.36 ± 1.08 | 3.10 ± 0.71 | 0.92 ± 0.51 | -12.3 ± 0.3 | 7 |
| TAMFQSVFERC | 4.26 ± 1.35 | 1.46 ± 0.05 | 0.34 ± 0.12 | -12.9 ± 0.2 | 5 |
| TAMFQSPFERC | 4.85 ± 2.35 | 1.55 ± 0.05 | 0.32 ± 0.17 | -13.0 ± 0.3 | 2 |
a Sequence replacements relative to WT are in bold and underlined.