Fig 3. Cytostatic activity of cell-free supernatant from jacaric acid and LPS-treated murine peritoneal macrophages on T-cell lymphoma MBL-2 cells.

Macrophages were pre-treated with different concentrations of jacaric acid (either 50 or 100 μM) in the presence of LPS (60 ng/ml) at 37°C for 72 h. Cells treated with 0.1% ethanol acted as the control. The pre-treated macrophages were washed and then incubated with fresh RPMI medium at 37°C for 48 h. Subsequently, the cell-free supernatant was added to the MBL-2 cells and further incubated at 37°C for 48 h. The cytostatic activity of the macrophages was then determined. The results were expressed as the mean percentage inhibition of cell proliferation ± SE. * p< 0.05.