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. 2015 Dec 2;10(12):e0143684. doi: 10.1371/journal.pone.0143684

Fig 6. Effect of N-acetyl-L-cysteine on the jacaric acid-induced ROS generation and cytostatic activity in murine peritoneal macrophages.

Fig 6

Macrophages were incubated with different concentrations of jacaric acid (either 50 or 100 μM) and N-acetyl-L-cysteine (either 10 or 100 μM), in the presence of LPS (60 ng/ml) at 37°C for 72 h. Cells treated with 0.1% ethanol acted as the control. (A) The intracellular levels of superoxide anion in the treated cells were measured by staining cells with DHE at 37°C for 30 min and analyzed for red fluorescence (FL-3) by flow cytometry. Results were expressed as mean ± SE. * p< 0.05; ** p< 0.01. (B) The pre-treated macrophages were incubated with MBL-2 cells at 37°C for 48 h and the cytostatic activity of macrophages was determined by the CyQuant® NF Cell Proliferation Assay Kit. The results were expressed as the mean percentage inhibition of cell proliferation ± SE. * p< 0.05; ** p< 0.01.