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. 2015 Dec 2;9(12):e0004238. doi: 10.1371/journal.pntd.0004238

Fig 1. L. major strain FV1 metacyclic promastigotes and LPG stimulate IL12B expression.

Fig 1

(A) Human DCs were infected with L. major FV1 or L. major LV39c5 parasite strains (n = 4 donors). After 8 hours, RNA was extracted from infected hDCs for cDNA generation and analyzed for IL12B expression by qRT-PCR. All values were significantly greater than uninfected. (B) Human DCs were infected with L. major FV1 metacyclic promastigotes (metacyclic), procyclic promastigotes (procyclic) or amastigotes (n = 3 donors). After 8 hours, RNA was extracted from infected hDCs for cDNA generation and analyzed for IL12B expression by qRT-PCR. All values were significantly greater than uninfected, except for amastigote infections. (C) Human DCs were exposed to different concentrations of LPG (0.5, 1, and 10 μg), derived from L. major FV1 metacyclic promastigotes (n = 4 donors). After 8 hours, RNA was extracted from infected hDCs for cDNA generation and analyzed for IL12B expression by qRT-PCR. Fold change was calculated utilizing the ΔΔCT method and depicted as fold change over uninfected samples. Box plots display the median value (line), the interquartile range (box), and Tukey whiskers encompassing data within 1.5 fold of the interquartile range. *Statistical significance as compared to uninfected control, (p<0.05). All values were significantly greater than uninfected.