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. 2015 Dec 2;9(12):e0004238. doi: 10.1371/journal.pntd.0004238

Fig 3. Kinetic analysis of IL12 and IL23 expression modulation by L. major LPG and PG null mutants.

Fig 3

Human DCs (n = 3 donors) were infected with L. major FV1 parasites: L. major FV1 (WT), LPG null mutant (FV1 lpg1 −), LPG add back (FV1 lpg1 −/+LPG1), PG null mutant (FV1 lpg2 −), or PG add back (FV1 lpg2 −/+LPG2). At 2, 4, 8, and 24 hours post infection, IL12B (A), IL12A (B), and IL23A (C) expression was measured by qRT-PCR. Fold changes were calculated using the ΔΔCT method and are represented as fold change over uninfected samples. Mean values of individual donors ± SD are presented. Statistical significance p<0.05, ANOVA with Bonferroni multiple comparisons test.