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. 2015 Dec 2;9(12):e0004238. doi: 10.1371/journal.pntd.0004238

Fig 7. The lpg1 − mutant affects IL12 associated gene regulator IRF8, and not IRF1.

Fig 7

Human DCs were infected with L. major FV1 parasites: L. major FV1 (WT), LPG null mutant (FV1 lpg1 −), LPG add back (FV1 lpg1 −/+LPG1), PG null mutant (FV1 lpg2 −), or PG add back (FV1 lpg2 −/+LPG2). At 8 hrs post infection, (A) IRF8 (n = 3 donors) and (B) IRF1 (n = 5 donors) expression was measured by qRT-PCR. Fold changes were calculated using the ΔΔCT method and are represented as fold change over uninfected samples. Box plots display the median value (line), the interquartile range (box), and Tukey whiskers encompassing data within 1.5 fold of the interquartile range. *Statistical significance (p<0.05). All values were significantly greater than uninfected.