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. 2015 Dec 2;10(12):e0143963. doi: 10.1371/journal.pone.0143963

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© 2015 Biner et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 6 — (A) Effect of temperature on CsHyal activity. (B) Thermostability of CsHyal measured at 4, 25, 37, and 50°C after 1, 3, 6, 12, and 24 h of incubation. (C) pH profile of CsHyal activity. (D) Determination of K_m and V_max using non-linear regression. (E) Effect of monovalent salts in 0.2 M NH₄ ⁺-buffer containing 0.2 M K⁺ or Na⁺, respectively. Activity decreased in Na⁺ compared to NH₄ ⁺ (* significance, p = 0.007). (F) Influence of divalent cations and EDTA on CsHyal activity.