Table 2. Specific amino acids for EV68 clade classification.

Clade	Position
	2	47	90	92	110	131	141	143	144	145	148	168	169	178	207	243	297	305
A	D	S	N	A	K	V	-	S	N	S	T	E	K	A	V	I	D	N
D	E	S	N	T	K	I	-	N	N	S	M	E	K	G	V	I	E	D
B	D	T	D	T	R	I	G	S	N	N	V	E	K	G	V	V	D	N
C	D	T	N	T	R	I	G	S	S	N	M	K	Q	G	I	I	D	N

Amino acids differing among the clades were compared based on an alignment of the full-length VP1 sequence performed using MEGA5 [36] with manual adjustment. Clade classification is shown in Fig 1. The numbers indicate residue position corresponding to the VP1 protein of the Fermon strain.