. Author manuscript; available in PMC: 2015 Dec 2.

Published in final edited form as: J Mol Biol. 2006 Feb 24;358(3):882–904. doi: 10.1016/j.jmb.2006.02.015

Table 3.

Hierarchical unfolding pathway by successively cleaving off fragments

Step	ΔG (kcal/mol)	T_m (K)	Fragmentation region
1	−1	366	92–95
2	1.4	372	8–10
3	0	372	1–7, 18, 21, 104–107
4	7	372	49–51

For the purpose of this Table, the quoted ΔG is taken at 340 K.