Table 1. Percentages of hES cell colonies exhibiting cells positive for DDX4.

Cell line/ stimulation	Cell colonies with no DDX4 expression	<25% DDX4-positive cells/colony	25%–75% DDX4-positive cells/colony	75%–100% DDX4-positive cells/colony
HS207/ None	5.8%	7.8%	20.4%	66.0%
HS207/ BMP7	27.3%	9.1%	30.3%	33.3%
HS360/ None	3.7%	6.7%	36.3%	53.3%
HS306/ BMP7	2.9%	8.8%	35.3%	52.9%
HS401/ None	7.3%	2.4%	20.7%	69.5%
HS401/ BMP7	37.8%	8.9%	13.3%	40.0%

Cell colonies were divided into four groups according to their expression profile: no DDX4 expression, <25%, 25%–75% or >75% of DDX4-positive cells present per cell colony. A pool of three experiments (minimum 45 cell colonies) for each cell line and condition was evaluated to define DDX4 expression.