Fig. 5. Reducing MSC-Substrate Adhesion by Blocking Surface Integrins with Free Peptide.
A. MSCs spread on 10 mM PA-PEG-RGD hydrogels with indicated concentrations of free RGD peptide in the culture media. (scale bar, 100 μm). B. Absolute and X velocities of MSCs on the gradient region of RGD gradient PA hydrogels with indicated concentrations of free RGD peptide in the culture media. (n > 40; mean ± S.E.M.; *p < 0.05). C. Mean displacement of particles embedded in PA hydrogels with 10 mM aPEG-RGD resulting from tractions of MSCs in normal media, and media supplemented with 0.02 mM free RGD peptide. (n > 10; mean ± S.E.M.; ***p < 0.005).