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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Cell Mol Bioeng. 2015 Jun 3;8(4):530–542. doi: 10.1007/s12195-015-0398-3

Fig. 6. Increasing Fibroblast-Substrate Adhesion by Increasing Surface Adhesive Ligand Density.

Fig. 6

A. A hydroxycoumarin dye (excitation 350 nm) pre-conjugated to aPEG-RGD allows for gradient characterization; incorporating 5.8 and 15.6 mM aPEG-RGD into two layers yield a gradient of approximately 10 mM/mm. B. Absolute velocities and X velocities of 3T3s on indicated regions of hydrogels with RGD concentration gradients spanning a 5.8–15.6 mM range. Low and high indicates cells found on the low and high static concentration regions of the substrate; gradient indicates cells found at the mixing interface of the substrate. Positive X velocity indicates migration towards regions of increasing adhesive ligand density. (n > 50; mean ± S.E.M.; n.s., not significant). C. Mean displacement of particles embedded in PA hydrogels with 5.8 and 15.6 mM aPEG-RGD resulting from tractions of 3T3s in normal media. (n > 10; mean ± S.E.M.; *p < 0.05).