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The effect of H2O2 on bacterial viability was assayed by counting the colony-forming units after exposing the cultures to 500 μM of H2O2 for 3.5 hours, in enriched BHI broth at 37°C in anaerobic conditions. Broken lines represent the numeration of viable cells after H2O2 addition (500 μM) and solid lines represent the control condition without H2O2. Wild-type (■), cydAB mutant (▲) and complemented cydAB mutant (●). Bars represent standard errors.
