Fig 2. DSF melting curves of TSVZV with dUMP.
(a) The DSF melting curve of apo-TSVZV has two melting transitions and two Tm can be estimated from the mid-slope of each sigmoidal transition. Dotted lines were added to the mid-slopes of each melting curve as references to the TSVZV Tm for each melting transition. (b) Increasing concentrations of dUMP only increased the Tm of the first melting transition. 100 μM of dUMP was sufficient to stabilize the first melting transition of TSVZV to produce a single melting transition. Further increase of the dUMP concentration could not increase the Tm beyond the second melting transition. (c) Raltitrexed further stabilized TSVZV in the presence of 100 μM of dUMP in the DSF. In the absence of dUMP, raltitrexed could not stabilize TSVZV in DSF.