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. 2015 Dec 2;10(12):e0143224. doi: 10.1371/journal.pone.0143224

Fig 3. Functional properties of vaginal Eomes+ NK cells and Eomes- ILC1-like cells.

Fig 3

(A) Bidimensional dot plots (left panel) show expression of CD27 and CD11b on gated Eomes+ NKp46+ CD3- cells from spleen, GLN and vaginas of naive C57BL/6 mice. Numbers in quadrants represent the percentage of cells for each subset. Histograms bars (right panel) represent results from five independent experiments expressed as mean values + SEM, n = 10 mice. **p<0.01, ***p<0.001, ****p<0.0001 Mann-Whitney U test. Spl.: spleen; GLN: genital lymph nodes; Vag.: vagina. Frequencies of CD107a+ and IFN-γ+ producing Eomes+ NK cells (B) and Eomes- ILC1-like cells (C) in cell suspensions from spleen, GLN and vagina of naive C57BL/6 mice. Cells (0.5 million/well) were stimulated in vitro for 4 hours with plate-bound isotype control (IC), anti NK1.1 mAb, PMA/ionomycin, IL-12 + IL-18 cytokines or YAC-1 tumor cells. CD107a and IFN-γ expression on cells were assessed by flow cytometry. Histogram bars represent results from three independent experiments expressed as mean percentages + SEM, n = 5 mice. **p<0.01, ***p<0.001, Mann-Whitney U test. (D) Bidimensional dot plots (left panel) show expression of TNFα and TRAIL on gated Eomes- ILC1 and Eomes+ NK cells from liver and vaginas of naive C57BL/6 mice after stimulation in vitro for 4 hours with PMA/ionomycin. Numbers in quadrants represent the percentage of cells for each subset. Results are representative of two independent experiments with n = 4 mice.