Fig 3. DEHP treatment causes enhanced proliferation rate, reduces lipid content, altered uptake of 2-deoxy-D [14C] glucose and similar palmitic acid uptake into 3T3-L1 adipocytes.
(A) BrdU incorporation in 3T3-L1 adipocytes. BrdU incorporation was assessed in 3T3-L1 preadipocytes grown on glass coverslips. Cells were induced to differentiate for 24 h, and then incubated with 10 μM BrdU for 3 h. (B) BrdU staining- BrdU-labeled cells were visualized using an anti-BrdU antibody and an Alexa Fluor® 488 anti-mouse antibody (C) Triglyceride content in mature 3T3-L1 cells (D) Oil Red O staining of 3T3-L1 adipocytes reveals reduced number of lipid droplet containing cells. 3T3-L1 cells were stained 8 days after induction (magnification 200x). (E) Basal and insulin stimulated uptake of 2-deoxy-D [14C] glucose was significantly increased in 3T3-L1 cells treated with DEHP. (F) Palmitic acid uptake in mature adipocytes with and without DEHP treatment. ** p<0.01, *** p<0.001.