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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Cancer Res. 2015 Nov 16;75(23):5093–5105. doi: 10.1158/0008-5472.CAN-15-0224

Figure 1.

Figure 1

CHK2 regulates androgen-dependent and androgen-independent prostate cancer cell growth. CyQuant Assay measured DNA content as a surrogate for cell number 7 days after shRNA transduction. Growth was compared to untreated empty vector control and the values were averaged across biological replicates. Error bars represent standard error of the mean. (A) Cell lysate was blotted for CHK2 and ERK1/2. Plotted is the CHK2 signal normalized to total ERK1/2. Quantitation was performed on Odyssey LICOR imaging system, n=3. The effect of two independent shRNAs on cell growth in LNCaP, VCaP, Rv1, and C42 cells in the absence or presence of 0.05nM R1881, n=5. (B) Knockdown of CHK2 enhances sensitivity to castration levels of androgen. The effect of two independent shRNAs on cell growth in LNCaP and Rv1 cells in the absence or presence of 0-0.05nM R1881, n=3. (C) The effect of two independent CHK2 shRNAs on cell growth in LNCaP and Rv1 cells in the presence of R1881 (0.05nM) and/or the anti-androgen MDV3100 (10μM). (D) Cell lysate was blotted for CHK2 and ERK1/2. Quantitation represents the CHK2 signal normalized to total ERK1/2, n=3. The effect of two independent shRNAs on cell growth in PC3 and DU145 cells in the absence or presence of 0.05nM R1881, n=3. Statistical analysis was performed using two-way ANOVA. * p<0.0001. (E) CHK2 knockdown has no effect on cell growth or AR transcription in non-AR expressing cells. The effect of two independent shRNAs on cell growth in LHS cells, n=3. Cell lysate was blotted for CHK2 and ERK1/2. Quantitation on Odyssey LICOR imaging system represents the CHK2 signal normalized to total ERK1/2, n=3.