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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Cancer Res. 2015 Nov 16;75(23):5093–5105. doi: 10.1158/0008-5472.CAN-15-0224

Figure 4.

Figure 4

CHK2 regulates AR transcriptional activity. Transcript levels of AR target genes in LNCaP, VCaP, or PC3 cells transduced with two independent shRNAs and pLKO control were measured by qPCR. RNA was isolated 2 hours following 1nM DHT exposure and 24 hours after addition of 1nM R1881. Transcript levels were normalized to the housekeeping gene, PSMB6, and compared to pLKO. Values were averaged across biological replicates +/− standard error of the mean, n=3. Shown are the histograms for (A) four immediate-early androgen-activated genes (NKX3.1, TMPRSS2, SGK1, and STAG1) and CHK2 in LNCaP cells. Statistical analysis was performed using two-way ANOVA. * p<0.05. (B) one androgen-activated genes (FKBP51) and CHK2 in VCaP cells. Statistical analysis was performed using two-way ANOVA. * p<0.05. (C) Table showing the fold changes in mRNA for six androgen-responsive genes (PSA, TMPRSS2, KLK2, SGK, ORM1, FST) in response to CHK2 knockdown and hormone treatment in LNCaP cells. Statistical analysis was performed using two-way ANOVA. * p<0.05 (vs pLKO, vehicle), ∧ p<0.05 (vs pLKO, 1nM R1881). (D) CHK2 knockdown in AR-null PC3 cells has no effect on AR transcriptional activity. Shown are the histograms for FKBP51 and CHK2.