Fig. 3. Influence of bevacizumab on apoptosis of retinal pigment epithelial (RPE) cells and B-cell leukemia/lymphoma (Bcl)-2 mRNA expression under low oxidative stress (100 µM H₂O₂). (A) Influence of bevacizumab on apoptosis of RPE cells under low oxidative stress (100 µM H₂O₂). The RPE cells were treated with various concentrations of bevacizumab (0, 0.33, 0.67, 1.33, and 2.67 mg/mL) under low oxidative stress (100 µM H₂O₂). Cell apoptosis was not significantly different at all concentrations of bevacizumab less than 100 µM H₂O₂. Each bar shows the mean ± standard deviation of results of three or more independent experiments. p < 0.05 vs. control. (B,C) Expression of Bcl-2 mRNA with bevacizumab under low oxidative stress (100 µM H₂O₂). Expression of Bcl-2 mRNA decreased under low oxidative stress (100 µM H₂O₂), and this decrease was proportional to the increase in bevacizumab dose. There was a statistically significant difference at all concentrations. The mRNA expression of Bcl-2 was normalized to glycerol-3-phosphate dehydrogenase (GAPDH, housekeeping gene). Each bar shows the mean ± standard deviation of results of three or more independent experiments. p < 0.05 vs. control.