Table 3.
EST-SSR primer pairs produced in EST (expressed sequence tags) sequencing database of Perilla frutescens.
| Number | Unigene ID | Repeat motifs | Left primer sequence | Tm. | Right primer sequence | Tm. | Product size (bp) | Annotation (NR DB) |
|---|---|---|---|---|---|---|---|---|
| 1 | Contig 17 | ATCAT(8) | GAGAGTATAAACAAATCCAAAACAGC | 58.795 | AGCCGGTATATCCAATTCCC | 60.006 | 562 | PREDICTED: protein CURVATURE THYLAKOID 1B, chloroplastic [Sesamum indicum] |
| 2 | Contig 67 | A(29) | AGCAACTGCGGGTAGCTAGA | 60.176 | CAATCCGACCACAGTTGATG | 59.96 | 172 | PREDICTED: photosystem I subunit O [Sesamum indicum] |
| 3 | Perilla-1-1a_pTriplEx2-seq_C16 | GA(16) | AGCGTACTGTTGAAAGCGTG | 59.148 | CAGCAAACGTGCTCGAATTA | 60.014 | 247 | PREDICTED: uncharacterized protein LOC105172991 isoform X2 [Sesamum indicum] |
| 4 | Perilla-1-1a_pTriplEx2-seq_E18 | CTT(9) | GCCAATTTGAAGCTTTAGCC | 58.969 | GAATGTGAAGTGGGAACGCT | 60.119 | 773 | PREDICTED: GRF1-interacting factor 3 [Sesamum indicum] |
| 5 | Perilla-1-1a_pTriplEx2-seq_M02 | AGAATG(4) | TGGAGCAAGTGAAGCAACAG | 60.175 | CCTTTTCAGTGAGGAGCCAG | 59.982 | 191 | |
| 6 | Perilla-1-2a_pTriplEx2-seq_A02 | TTTTG(7) | AATGATGGGTGTGATGAGCA | 59.925 | AAAGAATTTGAAGGCGCAGA | 59.96 | 401 | PREDICTED: homeobox-leucine zipper protein HAT5-like [Sesamum indicum] |
| 7 | Perilla-1-3a_pTriplEx2-seq_B13 | ATCAT(8) | GAGAGTATAAACAAATCCAAAACAGC | 58.795 | CGGTATATCCAATTCCCACG | 60.031 | 559 | hypothetical protein MIMGU_mgv1a015066mg [Erythranthe guttata] |
| 8 | Perilla-1-3a_pTriplEx2-seq_L05 | CT(14) | CCCAAATTCACATCCACTGA | 59.343 | AACAACTGACATGGCCTTCC | 59.973 | 185 | PREDICTED: uncharacterized protein LOC105160440 [Sesamum indicum] |
| 9 | Perilla-1-3a_pTriplEx2-seq_L15 | TC(15) | CAGTTTTAACTTCGCCTCGC | 60.018 | CACTCGCAAAAAGGGGTAAG | 59.741 | 619 | PREDICTED: annexin D5 [Sesamum indicum] |
| 10 | Perilla-2-1a_pTriplEx2-seq_A19 | GGA(8) | GCTCCTCGCAGTAACTTTGG | 60.015 | TCATCTCTTGCTCTGTTTCCA | 58.583 | 107 | hypothetical protein MIMGU_mgv1a016040mg [Erythranthe guttata] |
| 11 | Perilla-2-2a_pTriplEx2-seq_A06 | CT(12) | CATTGGCCTTAAACTTCGGA | 60.067 | ATAAATGTGGATTGGGGCAA | 60.016 | 341 | hypothetical protein MIMGU_mgv1a020048mg [Erythranthe guttata] |
| 12 | Perilla-2-2a_pTriplEx2-seq_C18 | AG(14) | GGGGGATCATTTCCAGTCTT | 60.133 | GTGCCCACTGGTTCTTTGTT | 60.012 | 404 | hypothetical protein MIMGU_mgv1a012334mg [Erythranthe guttata] |
| 13 | Perilla-3-2a_pTriplEx2-seq_E14 | GATGACGATGAT(2) | CTTTCCAACCCTCCGAATTT | 60.291 | CGACGCCTGTCTCATCTACA | 60.008 | 522 | NAC transcription factor 1 [Salvia miltiorrhiza] |
| 14 | Perilla-3-2a_pTriplEx2-seq_O22 | GA(17) | GGGGATATGTTATGTTGCTTGTT | 59.179 | TCGCCGTACTTGATCCCTAC | 60.096 | 514 | PREDICTED: uncharacterized protein LOC105169169 isoform X1 [Sesamum indicum] |
| 15 | Perilla-3-3a_pTriplEx2-seq_B03 | CT(16) | CGAGTGTGTTCGTATGGGTG | 60.025 | AACGCGTACGGAACAGAGAC | 60.321 | 184 | hypothetical protein MIMGU_mgv1a014836mg [Erythranthe guttata] |
| 16 | Perilla-3-3a_pTriplEx2-seq_B23 | TCCTCTTCCTCTCC(2) | TAGTGTCGAAGCTCAATGGC | 59.028 | TGACCAGCATCAGCTTTCAC | 59.992 | 662 | PREDICTED: chloroplast stem-loop binding protein of 41 kDa a, chloroplastic [Sesamum indicum] |
| 17 | Perilla-3-3a_pTriplEx2-seq_F11 | GAG(9) | GAAAGACTGGTTGGCTCTGG | 59.844 | ATCCAAAATTCGTCCTGTGC | 59.939 | 381 | hypothetical protein MIMGU_mgv1a011207mg [Erythranthe guttata] |
| 18 | Perilla-3-3a_pTriplEx2-seq_P03 | GA(13) | AAAGCTGTTTGCCCTTGCTA | 60.018 | CTCAAATGGAGTCACGCAGA | 59.984 | 284 | hypothetical protein MIMGU_mgv1a016830mg [Erythranthe guttata] |