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. 2015 Dec 3;5:17533. doi: 10.1038/srep17533

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Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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FACS-enriched CTC subsets derived from breast cancer patient cultured in Mammocult media^™ (StemCell Technologies, Inc.). CTC subsets grew as in vitro 3D CTC tumorspheres using stem cell and non-adherent conditions. White arrows indicated small-vesicle-like cells. Images were taken at 40X by phase contrast microscopy (Zeiss, Inc.). Representative images are shown.