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. 2015 Dec 3;5:17533. doi: 10.1038/srep17533

Figure 7. 3D invasion assays of in vitro 3D CTC tumorspheres.

Figure 7

(a) Experimental strategy with steps on 3D cell culture 96-well BME cell invasion assays; (b) Four CTC tumorspheres with breast cancer no brain metastasis were trypsinized and dissociated as single CTC units or pairlet cells. Control consisted of non-invasive MCF7 and invasive 231Br breast cancer cells. Images were captured at endpoint under 40× magnification using phase contrast microscopy (Zeiss, Inc.). Scale bars, 25 μM. Representative images of three independent experiments are shown.