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. 2015 Dec 3;5:17533. doi: 10.1038/srep17533

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Copyright © 2015, Macmillan Publishers Limited

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PBMCs subpopulation of breast cancer patient with and without brain metastasis were sorted by FACS. uPAR^+/− and int β1^+/− population were collected respectively (containing EpCAM-negative/CD45⁻/CD44⁺/CD24⁻expression markers). RNA were extracted, amplified and real-time PCR analysis were performed using RT²-PCR embryonic stem cell array profiler (Qiagen). The change in mRNA expression (>3 fold) is shown comparing uPAR⁺/int β1⁺ population to uPAR⁻/int β1⁻ population in CTCs isolated from patients clinically diagnosed with BCBM (a) or without BCBM (b).