Table 1. Relative expression of the VvCYP51 gene and tebuconazole sensitivity in 26 pB-Vv51wt transformants.
Isolate/transformant | Relative expression of the VvCYP51 genea | EC50 value | Resistance factor |
---|---|---|---|
FJ4-1b | 1.00 | 0.04 | |
UV10th | 55.04 ± 13.90 | 0.22 | 5.12 |
P-F 4 | 3.43 ± 0.82 | 0.11 | 2.67 |
P-F 7 | 8.70 ± 1.93 | 0.24 | 5.79 |
P-F 8 | 10.86 ± 6.50 | 0.13 | 3.10 |
P-F 9 | 20.90 ± 0.32 | 0.15 | 3.67 |
P-F 10 | 3.49 ± 1.87 | 0.12 | 2.90 |
P-F 13 | 2.84 ± 1.11 | 0.11 | 2.69 |
P-F 14 | 4.59 ± 0.58 | 0.28 | 6.64 |
P-F 15 | 15.74 ± 2.21 | 0.13 | 3.02 |
P-F 16 | 2.68 ± 1.06 | 0.13 | 3.17 |
P-F 17 | 4.35 ± 0.52 | 0.20 | 4.71 |
P-F 20 | 5.55 ± 0.46 | 0.27 | 6.50 |
P-F 22 | 10.49 ± 1.54 | 0.15 | 3.52 |
P-F 23 | 24.23 ± 6.76 | 0.20 | 4.76 |
P-F 25 | 8.25 ± 1.03 | 0.15 | 3.64 |
P-F 26 | 4.11 ± 2.67 | 0.18 | 4.26 |
P-F 28 | 3.86 ± 1.60 | 0.13 | 3.05 |
P-F 29 | 8.45 ± 2.55 | 0.26 | 6.07 |
P-F 31 | 3.13 ± 1.20 | 0.25 | 5.83 |
P-F 32 | 6.70 ± 0.03 | 0.32 | 7.71 |
P-F 33 | 4.14 ± 1.39 | 0.12 | 2.74 |
P-F 34 | 2.57 ± 1.79 | 0.15 | 3.55 |
P-F 36 | 9.50 ± 0.83 | 0.22 | 5.33 |
P-F 37 | 6.43 ± 2.84 | 0.11 | 2.52 |
P-F 38 | 5.76 ± 3.91 | 0.31 | 7.29 |
P-F 39 | 5.53 ± 2.33 | 0.13 | 2.98 |
P-F 40 | 6.88 ± 0.13 | 0.21 | 4.90 |
aThe expression of the VvCYP51 gene was normalized using α-tubulin gene expression levels and then compared to VvCYP51 expression in FJ4-1b. Data are shown as mean values ± standard errors.