Figure 1. Targeted Disruption of the Sec13 Gene in Mice Decreased Sec13 mRNA and protein Levels.

(a) Schematic representation of part of the Sec13 wild-type gene and the mutant allele. Exons 7, 8 and 9 were deleted using the Cre recombinase. (b) Genotyping was performed with primer sets that detect: (I) the wild-type allele and the hypomorphic allele containing the full construct including exons 7, 8, and 9, NEO cassette, FRT and loxP sites; (II) the allele deleted of exons 7, 8, and 9; (III) the wild-type allele and the allele deleted of exons 7, 8, and 9. (c) Sec13 mRNA levels were measured in wild-type and Sec13^H/− mouse lung fibroblasts by real time-RT PCR. GAPDH and actin were used as controls. (d,e) Sec13^+/+ and Sec13^H/− lung fibroblasts were subjected to immunofluorescence microscopy with anti-Sec13 antibodies (d) and immunoblot analysis with antibodies against the depicted proteins (e). (f) Immunoblot analysis of liver extracts from Sec13^+/+ and Sec13^H/− cells using antibodies against the depicted proteins. Immunoblots were quantified by ImageJ software.