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. 2015 Dec 3;5:17655. doi: 10.1038/srep17655

Figure 2. Nuclear Pore Complex Structure and Bulk Nucleo-Cytoplasmic Trafficking Are Not Significantly Altered in Sec13H/− Cells.

Figure 2

(a) Sec13+/+ and Sec13H/− lung fibroblasts were subjected to hypotonic treatment in order to expose nuclei, and prepared for FESEM imaging. Two large representative areas of nuclear envelopes are shown on the left along with a gallery of individual NPCs from each cell type. (b) Histogram shows a quantitative summary of the apparent external diameter measured only in NPCs embedded in flat membrane areas and viewed from the top. No significant architectural differences were detected between NPCs from Sec13+/+ or Sec13H/− cells. (c) Sec13+/+ and Sec13H/− lung fibroblasts were subjected to oligo-dT in situ hybridization to determine the intracellular distribution of poly(A) RNA. (d,e) Sec13+/+ and Sec13H/− lung fibroblasts were subjected to immunofluorescence microscopy with anti-HDAC1 antibody (d) or anti-hnRNP A1 antibody (e), respectively.