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. 2015 Dec 3;5:17742. doi: 10.1038/srep17742

Figure 4. Detection of infectivity in blood prior to onset of clinical disease.

Figure 4

Detection and quantification of infectivity in samples taken at 20, 40, 60, 80, 100, 120, 140 days post infection (dpi) and clinical end-point (EP) from CD-1 mice infected with RML prions was achieved using SSBA. Panel (A) shows the correlation between m value (n = 3, mean +/− SD) and titred RML-infected brain homogenate when spiked into either OFCS/10−4 FVB/N-Prnp0/0 (open circles) or control rodent blood (closed circles). Panel (B) shows the calculated LD50 units/ml in whole blood taken from mice culled at each of the 20 day intervals post inoculation with RML prions. The m value obtained in the SSBA for each of the time point groups (n = 12, mean +/− SD) was converted to LD50 units/ml using the correlation between m value and LD50 units/ml for titred RML-infected brain homogenate spiked into control rodent blood as shown in panel (A). The data were fitted to the equation m = (B + ((M*L)/(K + L))), where K = a saturation constant (mid-point of the m value amplitude), B = background m value and M = m value amplitude or range and L = prion titre.