Skip to main content
. 2015 Dec 3;5:17642. doi: 10.1038/srep17642

Figure 3. Az increases antibody production sufficiently by a single injection.

Figure 3

C57BL/6 mice were i.p. injected with KLH (100 μg/mouse) or OVA (100 μg/mouse). The immunized mice were i.p. injected with Az (2.25 mg/kg) once (one injection after antigen immunization), three (daily injections from 0 to 2 days after antigen immunization) and five times (daily injections from 0 to 4 days after antigen immunization). Boost immunization was performed by using the same protocol as prime immunization at 4 weeks after prime immunization. Sera were collected at the indicated times. Production of total IgG, IgG1 and IgG2c antibodies specific to (a) KLH or (b) OVA was determined by using an ELISA. All graphs show mean ± s.e.m. Data shown in (a,b) are representative of three independent experiments (n = 3–5 mice per group in each experiment). *p < 0.05; **p < 0.01.