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. 2015 Dec 3;5:17642. doi: 10.1038/srep17642

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Copyright © 2015, Macmillan Publishers Limited

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(a) Total IgG, IgG1 and IgG2c profiles against KLH or OVA were analyzed by determining the ratio of IgG2c/IgG1 by using sera collected at 6 weeks after prime immunization (mean ± s.e.m). (b) For titration of antibody levels produced after OVA immunization, sera collected at 1–6 weeks after immunization were serially diluted and total IgG, IgG1 and IgG2c levels for OVA were determined (mean ± s.e.m.) (●; No adjuvant, ; Alum, ; Az, ; PolyI:C). (c) C57BL/6 mice were i.p. injected with Az (2.25 mg/kg) and the sera was collected at the indicated times. The amounts of cytokines were determined by using a multiplex cytokine assay. Data are representative of three independent experiments (n = 3–5 mice per group in each experiment). ^*p < 0.05; ^**p < 0.01; ^***p < 0.001.

Inline graphic — (a) Total IgG, IgG1 and IgG2c profiles against KLH or OVA were analyzed by determining the ratio of IgG2c/IgG1 by using sera collected at 6 weeks after prime immunization (mean ± s.e.m). (b) For titration of antibody levels produced after OVA immunization, sera collected at 1–6 weeks after immunization were serially diluted and total IgG, IgG1 and IgG2c levels for OVA were determined (mean ± s.e.m.) (●; No adjuvant, ; Alum, ; Az, ; PolyI:C). (c) C57BL/6 mice were i.p. injected with Az (2.25 mg/kg) and the sera was collected at the indicated times. The amounts of cytokines were determined by using a multiplex cytokine assay. Data are representative of three independent experiments (n = 3–5 mice per group in each experiment). ^*p < 0.05; ^**p < 0.01; ^***p < 0.001.