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. 2015 Dec 3;5:17622. doi: 10.1038/srep17622

Figure 2. Vaccination with exosomes from OVA-loaded and poly(I:C)-activated DCs strongly activates proliferation and acquisition of effector functions of adoptively transferred OT-I OVA-specific CD8+ T cells in vivo.

Figure 2

(a) CFSE-labeled OT-I CD8+ T cells (CD45.2+) were intravenously transferred into CD45.1+ recipient mice on day 0. Recipient mice were vaccinated on day 1 with 50 μg of the indicated Dexo formulation or saline. On day 6, spleens and LNs were collected to analyze OT-I cells. (b) Proliferation was measured by flow cytometry as dilution of CFSE dye in adoptively transferred CD45.2 + CD3ε +CD8α+ OT-I cells retrieved from the spleen (left) and LNs (right). (c) Acquisition of the CD62L-CD44+ effector memory phenotype of adoptively transferred CD45.2+ CD3ε+ CD8α+ OT-I cells was measured by flow cytometric analysis of cells harvested from the spleen (left) and LNs (right). Data represent mean ± SEM from 2 independent experiments (N = 10). Statistical analysis was performed by one-way ANOVA and Bonferroni post-hoc test correction. In (b) statistics represent comparisons between Dexo(OVA + pIC) i.v. with both Dexo(OVA + LPS) i.v. and Dexo(OVA + CpGB) i.v. groups or between Dexo(OVA + pIC) i.d. with both Dexo(OVA + LPS) i.d. and Dexo(OVA + CpGB) i.d. groups. In (c) statistics represent comparisons between the indicated experimental groups. *P < 0.05 **P < 0.01 and ****P < 0.0001.