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. 2015 Dec 3;5:17806. doi: 10.1038/srep17806

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(A) Ectopic bnl expression (dppGAL4UAS-GFP; UAS-bnl) (green) in the wing disc induced new tracheoblasts expressing high levels of vn (red). (B) vnlacZ expression (red) in random flip out clones in the tracheal system expressing either tauGFP or constitutively active Btl (Tor-Btl). Note that the over-activation of FGF/Btl signaling in ASP increased the number of tracheoblasts and the levels of vnlacz. (C-H) Flip out clones generated in the tracheal system expressing UAStauGFP (C), UASDER^DN (D), UASvn^RNAi (E), UASvn (F), UAStor-btl (G) and UAStor-btl and UASvn^RNAi (H). Note the reduction of clone size in (H) compared to (G). (I) Quantification of clone volume. Note that overexpression of vn^RNAi reverted the overgrowth produced by the over-activation of FGF/Btl signaling. The number of clones analyzed is indicated in the histogram.