Table 1. Peptide designs.

Names, sequences, mean residue ellipticities at 222 nm (MRE₂₂₂) and 5 °C, corresponding percent helicities, and midpoints of thermal denaturation curves (T_m) for the peptides used in this study. Conditions: phosphate buffered saline (PBS, 137 mM NaCl), pH 7.4, and 100 μM peptide concentration. The purity and identities of the peptides were confirmed by HPLC and MALDI-TOF mass spectrometry (Supplementary Figs. 1 – 27).

Peptide Name	Sequence1	MRE222	Fraction Helix (%)	Tm (°C)
(E2K2)6	Ac–GEEKKEEKKEEKKEEKKEEKKEEKKGYY–NH2	813	−1
(EK)12	AC–GEKEKEKEKEKEKEKEKEKEKEKEKGW–NH2	−8,136	22
(KE)12	AC–GKEKEKEKEKEKEKEKEKEKEKEKEGW–NH2	−4,180	12
(E4K4)4	AC–GEEEEKKKKEEEEKKKKEEEEKKKKEEEEKKKKGW–NH2	−36,454	94
(E4K4)3	AC–GEEEEKKKKEEEEKKKKEEEEKKKKGW–NH2	−27,885	74
(E4K4)2	AC–GEEEEKKKKEEEEKKKKGW–NH2	−23,374	65
(E4K4)	AC–GEEEEKKKKGW–NH2	−4,219	14
(K4E4)4	AC–GKKKKEEEEKKKKEEEEKKKKEEEEKKKKEEEEGW–NH2	−35,479	91	28
(K4E4)3	AC–GKKKKEEEEKKKKEEEEKKKKEEEEGW–NH2	−23,493	62
(K4E4)2	AC–GKKKKEEEEKKKKEEEEGW–NH2	−7,666	22
(K4E4)	AC–GKKKKEEEEGW–NH2	816	−1
A4(E4K4)3A4	AC–GAAAAEEEEKKKKEEEEKKKKEEEEKKKKAAAAGW–NH2	−33,934	87
A4(E4K4)2A4	AC–GAAAAEEEEKKKKEEEEKKKKAAAAGW–NH2	−27,313	72
A4(E4K4)A4	AC–GAAAAEEEEKKKKAAAAGW–NH2	−18,885	53
A4(K4E4)3A4	AC–GAAAAKKKKEEEEKKKKEEEEKKKKEEEEAAAAGW–NH2	−38,166	98	33
A4(K4E4)2A4	AC–GAAAAKKKKEEEEKKKKEEEEAAAAGW–NH2	−29,687	78	21
A4(K4E4)A4	AC–GAAAAKKKKEEEEAAAAGW–NH2	−21,847	61
A4(E4K4)3	AC–GAAAAEEEEKKKKEEEEKKKKEEEEKKKKGW–NH2	−33,288	87
(E4K4)3A4	AC–GEEEEKKKKEEEEKKKKEEEEKKKKAAAAGW–NH2	−32,668	85
A4(K4E4)3	AC–GAAAAKKKKEEEEKKKKEEEEKKKKEEEEGW–NH2	−35,177	91	25
(K4E4)3A4	AC–GKKKKEEEEKKKKEEEEKKKKEEEEAAAAGW–NH2	−32,796	85	22
A4(E4K4)A4(E4K4)A4	AC–GAAAAEEEEKKKKAAAAEEEEKKKKAAAAGW–NH2	−31,779	83	24
A4(K4E4)A4(K4E4)A4	AC–GAAAAKKKKEEEEAAAAKKKKEEEEAAAAGW–NH2	−37,155	97	29
(E3K3)4	AC–GEEEKKKEEEKKKEEEKKKEEEKKKGWW–NH2	−25,428	67
(K3E3)4	AC–GKKKEEEKKKEEEKKKEEEKKKEEEGWW–NH2	−12,229	33
A4(E3K3)4A4	AC–GAAAAEEEKKKEEEKKKEEEKKKEEEKKKAAAAGWW–NH2	−31,869	82	21
A4(K3E3)4A4	AC–GAAAAKKKEEEKKKEEEKKKEEEKKKEEEAAAAGWW–NH2	−30,287	78	14

¹Amino acids are represented by standard one-letter codes: A, alanine; E, glutamic acid; G, glycine; and K, lysine. To remove complicating terminal formal charges, and to ameliorate end effects in the helices, the E/K regions in all of the peptides for this study were flanked by glycine residues, and capped with acetyl (Ac) and amide groups (NH₂) at their N-and C-termini, respectively. C-terminal tryptophan (W) or tyrosine (Y) residues were included for concentration determination.