Table 1.
RPA primers and probes designed in this study
| Name | Sequence (5’ –3’) | Genome location(U33419.1) |
|---|---|---|
| ORFVRPA F1 | CTAGTAAGCTGTTCGAGATCACCTTGTTCATCATG | 4465-4499 |
| ORFV RPA F2 | TTCCGACGGACGTATGAATATGTCCATGGTGAACG | 4530-4564 |
| ORFV RPA F3 | CGTATGAATATGTCCATGGTGAACGATGTACCAAC | 4540-4574 |
| ORFV RPA R1 | AGCGTTCATTCAATTCATGTCTGAGGTAAACGGCA | 4702-4736 |
| ORFV RPA R2 | GACCACGTAAAAGTGGTGTTCGAAAAACTTCACAA | 4651-4685 |
| ORFV RPA R3 | GGTAAACGGCAATGATGTTCGTGACAAAGACCACG | 4679-4713 |
| ORFV RPA P | AACGTATCCCATGCAGTAAAGCATAGTCCG | 4582-4631 |
| (FAM-dT)C(THF)C(BHQ1-dT)TATAAACTCAGGAAC-p |
ORFV RPA F and R, RPA primer; ORFV RPA P, RPA Exo probe; BHQ1-dT, thymidine nucleotide carrying Black Hole Quencher 1; THF, tetrahydrofuran spacer; FAM-dT, thymidine nucleotide carrying fluorescein; P (phosphate), block elongation