Table 4.
Odds ratio for colorectal cancer by combined mutation status of KRAS and BRAF according to plasma adiponectin level in the combined cohort of the Nurses’ Health Study and the Health Professionals Follow-up Study
| Colorectal cancer subtype | Tertile 3 | Tertile 2 | Tertile 1 | P trend* |
|---|---|---|---|---|
| Combined (women [NHS] and men [HPFS]) | ||||
| KRAS wild-type & BRAF wild-type | ||||
| No. of case patients/control individuals (130/251) | 37/79 | 45/87 | 48/85 | |
| Age-adjusted OR (95% CI)† | 1 (referent) | 1.13 (0.66 to 1.94) | 1.32 (0.73 to 2.38) | .32 |
| Multivariable-adjusted OR (95% CI)‡ | 1 (referent) | 1.06 (0.60 to 1.90) | 1.07 (0.56 to 2.04) | .79 |
| KRAS-mutant & BRAF wild-type | ||||
| No. of case patients/control individuals (133/255) | 30/89 | 45/86 | 58/80 | |
| Age-adjusted OR (95% CI)† | 1 (referent) | 1.58 (0.90 to 2.78) | 2.41 (1.33 to 4.39) | .006 |
| Multivariable-adjusted OR (95% CI)‡ | 1 (referent) | 1.87 (1.02 to 3.40) | 2.93 (1.54 to 5.56) | .002 |
| KRAS wild-type & BRAF-mutant | ||||
| No. of case patients/control individuals (41/81) | 18/28 | 8/22 | 15/31 | |
| Age-adjusted OR (95% CI)† | 1 (referent) | 0.59 (0.22 to 1.55) | 0.77 (0.33 to 1.81) | .44 |
| Multivariable-adjusted OR (95% CI)‡ | 1 (referent) | 0.54 (0.19 to 1.52) | 0.53 (0.21 to 1.36) | .15 |
*Tests for trend (the Wald statistics, two-sided) were conducted using the median values for each tertile of plasma adiponectin. CI = confidence interval; HPFS = Health Professionals Follow-up Study; NHS = Nurses’ Health Study; OR = odds ratio.
† Results were based on conditional logistic regression analysis with adjustment for age at blood draw and date of blood collection.
‡ Results were based on conditional logistic regression analysis (which accounted for age at blood draw and date of blood collection) with additional adjustment for fasting status (<8 hours, ≥8 hours since last meal), body mass index (continuous, kg/m2), physical activity level (continuous, metabolic equivalent hour per week), family history of colorectal cancer (yes, no), multivitamin use (yes, no), regular aspirin use (yes, no), hormone replacement therapy (yes, no; NHS only), history of previous lower endoscopy (yes, no), pack-years of smoking before age 30 years (continuous), intake of total calorie (continuous, kcal/day), red meat intake (continuous, serving/day), processed meat intake (continuous, serving/day), calcium intake (continuous, mg/day), folate intake (continuous, μg/day), alcohol consumption (continuous, g/day), and plasma 25-hydroxyvitamin D (tertile, ng/mL). The median (range) levels of plasma adiponectin across tertiles among controls are the same as shown in Table 2.