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. 2014 Oct 21;1:20. doi: 10.3389/fvets.2014.00020

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Copyright © 2014 Roussey, Steibel and Coussens.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Relative mRNA abundance of select genes in CD4⁺CD25⁻ T cells cultured with nil- or MAP-infected MDMs. CD4⁺CD25⁻ T cells were cultured with 4-day old MDMs that were either nil- or MAP-infected. Non-adherent cells were collected following 18-hour incubation. Subsequent RNA extraction and qPCR results are shown. Subclinical infected animals showed up-regulation of a variety of immune genes including those encoding both Th1 (IFNγ, IL1A, TBX21) and Th2 (IL10, IL4) cytokines, whereas clinical infected animals showed no significant changes. n = 3–8/group, *p < 0.05.