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. 2014 Oct 21;1:20. doi: 10.3389/fvets.2014.00020

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Copyright © 2014 Roussey, Steibel and Coussens.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Relative mRNA abundance of select genes considering the effect of adding MAP to heterogeneous PBMC cell cultures. When disregarding Johne’s test status or the addition of expanded Tregs (these differing culture conditions were pooled in this analysis), the addition of live MAP resulted in an increase in the relative mRNA abundance of several genes including the Treg-associated FOXP3. mRNAs encoding numerous Th1- (IFNγ, IL1A, TBX21, TNFA) and Th17-associated (IL23, IL17A, IL6) proteins were significantly or near-significantly up-regulated. n = 3–8/group, *p < 0.05.