Table 1.
qPCR primers.
| Gene | Forward Primer (5′ → 3′) | Reverse Primer (5′ → 3′) |
|---|---|---|
| CTLA4 | CTGTGCTGGGACCTACATGG | TTCCTCTGGAGGTGCCAATG |
| FOXP3 | CACAACCTGAGCCTGACAA | TCTTGCGGAACTCAAACTCATC |
| GAPDH | AGGAGCACGAGAGGAAGAGT | TTCTCAGTGTGGCGGAGATG |
| GATA3 | AGGTACGTCCTGTGCAAACT | AGACAGGGTCTCCATTGGCA |
| GITR | CCTGTTTCCCGGAAACAAGAC | CCAGAGAGAGGATGACGATGGT |
| IFNγ | TCTGGTTCTTATGGCCAGGG | GAAGAGAGGCCCACCCTTAG |
| IL1A | TTGGTGCACATGGCAAGTG | GCACAGTCAAGGCTATTTTTCCA |
| IL10 | GCTGTATCCACTTGCCAACC | ATCCAGCAGAGACTGGGTCA |
| IL12P40 | CACCCCGCATTCCTACTTCT | TGGCATGTGACTTTGGCTGA |
| IL17A | CATCTCACAGCGAGCACAAG | CCACCAGACTCAGAAGCAGT |
| IL2 | CAAGCTCTACGGGGAACACA | TAGCGTTAACCTTGGGCACG |
| IL23 | AGCTCTCACAGCAACTCTGC | TGTCCCATTGGTAGGTGTGC |
| IL4 | GGCGGACTTGACAGGAATCT | TTGTGCTCGTCTTGGCTTCA |
| IL6 | GGCTCCCATGATTGTGGTAGTT | GCCCAGTGGACAGGTTTCTG |
| PFN1 | CATGGACCACGGTCTCTTGAA | GGTGAGGCAAGCATTTGACC |
| RORC | GAGTTCGCTAAGAGGCTCCC | TCCATGGCTCCTGCTTTGAG |
| TBX21 | ACCACCTGTTGTGGTCCAAG | ATCCGGTAATGGCTGTTGGG |
| TGFβ1 | CGAGCCCTGGACACCAACTAC | CCGGAAGTCAATGTAGAGCTGA |