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. 2014 Oct 20;1(1):91–102. doi: 10.1093/rb/rbu011

Figure 3.

Figure 3.

(A) Schematic illustration of the fabrication of micropatterned temperature-responsive PIPAAm/PIPAAm-b-PAcMo patterned brush surface via a two-step RAFT polymerization. PAcMo have been grafted from the terminal of PIPAAm chains via reactive DTB groups. (B) Phase contrast image shows that NHDFs only adhere on 100 µm-wide PIPAAm regions of PIPAAm/PIPAAm-b-PAcMo patterned surface after 24 h culture. (C) Phase contrast image and (D) fluorescence image show that NHDF sheet is formed on a 50-µm-wide PIPAAm/PIPAAm-b-PAcMo patterned surface after a 5-day culture. (D) Actin and nuclei of NHDFs are stained with AlexaFluor568-phalloidin (red) and Hoechst 33258 (blue), respectively. Scale bars: 100 µm. (Reprinted with permission from Ref. [78] © 2011 the American Chemical Society.)