Figure 6.

(A–C) Live/dead assay at 7 days. 2 × 10⁵ cells were seeded onto control and 1% scaffolds (425–600 µm in diameter) and cultured for 7 days in growth medium to determine whether cells could adhere only to nanofibers alone. (A-left) The controls scaffolds, viable cells shown in green adhere solely to the surface of the microspheres (scale bar 100 µm). (B-middle) The pore space of a nanofiber-permeated pore space in a sintered microsphere scaffold. Cells appear to adhere not only to the surface of the microspheres but also to nanofibers. The detail of the pore space shown in C is indicated with a yellow box (scale bar 100 µm). (C-right) The detail of the pore space of a hybrid scaffolds showing a viable cell on nanofibers indicated with a yellow arrow (scale bar 10 µm).