Figure 7.
DNA content assay; 2 × 105 MC3T3 cells were seeded onto scaffolds and cultured for 21 days in growth medium. At 1, 3, 7, 14 and 21 day time points, the scaffolds were evaluated for DNA content. Although these data showed that the indirect marker for cell proliferation, DNA, increased from day 1 to day 21, there were no statistical differences in proliferation between groups. These results indicate that the presence of nanofibers in the pore spaces of the scaffold do not inhibit the underlying proliferative capacity of cells seeded on the sintered microsphere matrix.