Table 1.

Ratio of molecules of cross-linking agents to collagen carboxylic acid groups

Cross-linking condition	Solution concentration (EDC, NHS)	Ratio of molecules EDC to number of carboxylic acid groups	Ratio of molecules NHS to number of carboxylic acid groups
25 mM	25 mM, 12.5 mM	70:1	28:1
2.5 mM	2.5 mM, 1.25 mM	7:1	2.8:1
0.25 mM	0.25 mM, 0.125 mM	0.7:1	0.28:1