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. 2015 Dec 3;11(12):e1005307. doi: 10.1371/journal.ppat.1005307

Fig 3. LAI-1-dependent inhibition of cell migration requires IQGAP1 and Cdc42.

Fig 3

(A) Confluent cell layers of A549 epithelial cells were treated with siRNA against IQGAP1, Cdc42, RhoA or Rac1 for 2 days. The cells were then treated or not with LAI-1 (10 μM, 1.5 h), scratched and let migrate for 24 h. Prior to imaging (0, 24 h), the detached cells were washed off. (B) The scratch area was quantified using ImageJ software at 7 different positions per condition in triplicate samples. Means and standard deviations of triplicate samples are shown (**p < 0.01). The data is representative of 3 independent experiments.