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. 2015 Dec 3;10(12):e0144100. doi: 10.1371/journal.pone.0144100

Fig 3. Pancreatic Progenitors in ARX ko hESCs.

Fig 3

(A) Immunostaining of PDX1 (green) and NKX6.1 (red) in agarose-embedded wild type (WT) and ARX knockout (ARX ko) cell sheets from day 14, 17, 20, and 26 of culture. * indicates region of enlargement (right) which includes individual red and green channels and overlay with DAPI (blue) showing coexpression patterns. (B) Immunostaining of 13 week human fetal pancreatic tissue the same as A. (C) RT-qPCR of day 17 and day 26 whole population samples examining the expression of transcription factors believed to be involved in pancreatic progenitor induction. Wild type (black bars) and ARX ko clone 1 (red bars) cell samples relative to adult human islets expression levels. † indicates p < 0.05 within the genotype over time, * indicates p < 0.05 wild type vs ARX ko at given culture day, N = 4 per group. (D) Immunostaining of NKX6.1 (green), proliferating cell nuclear antigen (PCNA, red), and chromogranin A (blue) in 26 day differentiated wild type and ARX ko cells, 13 week human fetal pancreatic tissue, and adult human pancreatic tissue. Inset is a ~3x enlarged portion from the region indicated by the star. Scale bar is 100 μm for all panels.